FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35888521
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373490.10406948784543113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT76350.016.1897294
CGACCTA36100.016.1438054
TAGGTCG18850.013.4639913
CATTCGG66700.013.3176434
GCATTCG67550.013.2196413
TTAGCGG31750.013.0265789
CTCGATT65350.012.15449848-49
TACGCAC57350.011.4732389
CTTAGCG35000.011.4141178
ACGCACG45450.011.27159152-53
TTCTCGA70150.010.95434346-47
GTACGCA48000.010.86865750-51
ACGGTCG21450.010.73646794
TACCGGT30550.010.61534544-45
CGACCGT29050.010.5237171
GTCGTAA23950.010.4006815
GCGAACG29250.010.28360753
CGGCGAA37600.010.0070031
CCGGTAC59750.09.12465260-61
ATTCCGT85400.09.10820352-53
Produced by FastQC (version 0.11.5)