FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28090897
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354870.12632918058828807No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT284370.10123208240733644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT105100.024.19296894
TCACCCG128100.021.7936693
ACCCGGC150450.018.9934755
CTCGATT90950.018.65513248-49
CACCCGG161750.017.7246884
TTCTCGA97550.017.4411546-47
TAGCATA106250.017.384289
ATTCCGT98400.017.26657752-53
ACGCACG65350.017.00899352-53
GTAGCAT110900.016.9096418
GTACGCA67200.016.68063450-51
CCAGTAG121100.015.0584615
CGTTCTT108600.015.03918276-77
CGGCCCG207000.014.6447648
TACGCAC71550.014.61550450-51
CGACCTA22950.014.3353534
ATAGCTC120000.014.33489238-39
CCGGTAC79550.014.297894560-61
TCTCGAT99500.014.029016548-49
CGGTACA76600.013.68273760-61
Produced by FastQC (version 0.11.5)