FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28090897
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41900.022.4900348-49
TCGTATG32000.021.88396544-45
CGCGTAT11700.021.0893544-45
CGTATGC52300.018.15262446-47
CTCGTAT43150.016.28358844-45
AACGTTC59800.016.0724734-35
ATCGCGT17100.015.11667642-43
CGCTAAT10900.013.376961
TCGACCG33450.013.34833930-31
TTACGGA40400.013.2621528
GTATGCC73550.013.16358846-47
CACAACG75600.013.14886830-31
CAACGTT75850.013.04326332-33
GTTACGG36550.012.9874687
AGTTACG39650.012.9203356
GTTCGAC35300.012.71544428-29
ACAACGT82100.012.47966932-33
CGTATCG40500.012.4170733
CGGCTAT34100.012.2760281
CCCGTAT44200.012.1312291
Produced by FastQC (version 0.11.5)