FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32818563
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37450.017.13052448-49
CGCGTAT13550.016.4757144-45
TATCGCG13600.016.4151540-41
ATCGCGT16150.014.40530542-43
TCGTATG30600.013.43929444-45
CGTATGC46900.013.42832446-47
CCCGTCT156400.013.2919011
CGTATCG37250.013.2481173
CCCGTAT54000.012.5421011
GTATCGA33600.012.3093344
CGCTAAT13450.012.239041
CGCTAGT12350.012.1866561
CCGTATC60900.011.0370642
CGCTATT22650.010.7978361
TCTACTA212050.010.239887
CTCGTTT60000.010.1904571
GTCCGAT33550.09.9533071
CTCGTAT44300.09.86663444-45
CCGGCAT70600.09.8595841
CCCGTTT79250.09.8516821
Produced by FastQC (version 0.11.5)