FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32818563
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370900.11301530783051045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG23650.014.3085443
CGACCTA29500.012.9049724
GCGATTT61300.012.72745894
CATTCGG56700.012.5995384
GCATTCG56600.012.2896453
CGGCGAA38850.010.8945751
TTAGCGG27650.010.3688079
ACGCACG36000.09.72630452-53
CGACCGT28300.09.6382981
CTCGATT54600.09.59791748-49
GTACGCA38550.09.57061150-51
CGCGAGT45000.09.5101571
TAGGTCG16850.09.4835973
TACGCAC43700.09.4644489
CGCAAAA101650.09.2992421
CGGGGTT99000.09.0731281
GAACGCG43050.08.9523035
GGCGAAC39950.08.941332
TTCTCGA59550.08.91849246-47
CTTAGCG30800.08.8505738
Produced by FastQC (version 0.11.5)