FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31593272
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCATCTCGTAT383820.12148789147258948TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG82000.035.6792348-49
CGCGTAT31750.033.7505744-45
CGTATGC93550.031.24902746-47
TCGTATG61700.030.65989544-45
ATCGCGT36250.030.1442842-43
GTATGCC116650.025.46374546-47
CTCGTAT75400.025.33839844-45
GCGTATG49750.021.82271844-45
TCGCGTA54250.020.01253942-43
ATGCCGT162050.018.43132248-49
GCCGTCT154550.018.30700950-51
CGTATCG57050.015.8997973
CCCGTAT62900.015.7034161
TGCCGTC196500.015.33152750-51
GTATCGA47600.015.1068674
ATCTCGT131900.015.07250842-43
CCGTCTT185750.014.87779152-53
GAGCACA435550.014.7941289
ACGTCTG218850.014.51786714-15
CGGAAGA442600.014.4310524
Produced by FastQC (version 0.11.5)