FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31581678
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG497720.15759770585970764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT72950.013.78738394
CGCAAAA101800.013.3986181
GCGAACG17700.013.2766993
CGACCGT26900.011.8895781
CGGCGAA30300.011.797251
CTCGATT58500.011.6896648-49
CCGTCAT73800.011.0255321
ATTCCGT63100.010.61399652-53
CTAACCC120600.010.600248
GTACGCA38250.010.38290850-51
TTCTCGA66550.010.27567646-47
ACGCACG39550.010.04161652-53
TACTCTA81650.09.5553369
TCACCCG143850.09.5403953
GTAGGTC57750.09.4409542
ACCCGGC136300.09.3447315
TAACCCC134900.09.3372069
CGGCCCG142250.09.1851578
CGTTCTT70200.09.17227176-77
GCGCTAA52150.08.96733942-43
Produced by FastQC (version 0.11.5)