FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33860689
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA35900.019.8995174
TACGCAC49700.015.5088969
GCGAACG26100.014.0458333
CATTCGG57250.013.5457124
CGGCGAA36500.013.1424181
GCATTCG55900.013.0320673
TTAGCGG33350.011.6970319
GTCGTAA23750.011.6756695
CTTAGCG35850.011.5368418
CGACCGT25750.010.5929941
TGACGAC77350.010.5792991
GCGATTT58200.010.01367394
GGTCGTA35300.09.852594
TAGGTCG19250.09.7661343
GGCGAAC40750.09.6885872
TCGTAAG31300.09.3098096
AGTCGCG31050.09.23346
GAACGCG39500.09.1619315
CGGGGTT121200.09.1187061
TACCGGT33300.09.10352344-45
Produced by FastQC (version 0.11.5)