FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33880026
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG341750.10087064277931782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA36850.019.6415564
TACGCAC50750.014.4471399
GCGAACG25200.013.9879273
CATTCGG57200.013.804034
GCATTCG57350.013.1942623
CGGCGAA38700.011.4247021
CTTAGCG35000.011.41416558
TTAGCGG35450.011.40185459
GTCGTAA23500.011.1998665
TAGGTCG20100.010.9899183
TACCGGT33900.09.91293344-45
TAATTCG24950.09.79548114-15
GGCGAAC40050.09.7406832
TGACGAC77900.09.7211231
GACGACC97250.09.4245112
TCGTAAG33500.09.2595916
GGTCGTA35750.09.071224
ACGACCT94500.08.9025383
GCGATTT62450.08.88059894
CGACCGT26200.08.7967611
Produced by FastQC (version 0.11.5)