FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30206172
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53700.028.26953948-49
CGCGTAT19900.027.86887644-45
ATCGCGT21400.026.46450642-43
TCGTATG38200.025.28360444-45
CGTATGC64800.023.68092746-47
GTATGCC84600.018.30524446-47
CTCGTAT54900.017.8922344-45
GCGTATG34500.016.62000844-45
GTATCGA40050.015.8424814
CGTATCG43550.015.5405463
AGTCACG108100.015.39135128-29
ACGTCGG123650.013.72156332-33
CCCGTAT62350.013.5024321
CGCTAAT11800.013.1530761
TCGGAGC129050.012.76499134-35
GTCACGT131300.012.70757728-29
GCCGTCT121050.012.46323350-51
ATGCCGT129350.012.35386948-49
CCGTATC67500.012.2559862
CACGTCG137750.012.07835930-31
Produced by FastQC (version 0.11.5)