FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30231317
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346640.11466255340447126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT66250.019.15454794
TACCGGT76700.016.05469544-45
CTTAGCG25600.015.238078
GTTACCG85300.014.71142642-43
GTGTCGC86400.014.44250812-13
CCGGTAT87800.014.13206246-47
TAGGTCG16000.014.0998043
CTCGATT56900.013.7117148-49
TTAGCGG29050.013.5901739
ACCGGTA83600.013.4646644-45
CGGTATT86050.013.16322546-47
TCACCCG112050.013.1287783
CGTTGTT95950.013.12757638-39
TTACCGG87400.012.93290842-43
TTCTCGA63250.012.37228346-47
AGCCGTA94400.012.023787562-63
GCGAACG17900.011.8154783
TAGCATA79250.011.8017289
ACGCACG41450.011.79239652-53
AGCGCTC90200.011.7237849
Produced by FastQC (version 0.11.5)