FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37825863
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA448600.08.60153694
AGATCGG510000.08.27557894
ATCGGAA423700.08.11978994
TCGGAAG442350.07.79869994
TAGATCG157700.07.420954794
ACACGTC182400.07.085981494
CGGAAGA424750.07.01532894
CGTCTGA137300.06.230070694
ACGTCTG176750.05.95635794
CAGATCG158800.05.919317294
CACACGT229100.05.867229594
CACGTCT205650.05.713515894
AAGATCG222250.05.4982494
GCACACG264200.05.443531594
AGAGCAC445300.05.351153494
Produced by FastQC (version 0.11.5)