FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37825863
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA447100.08.58884794
GTCGTGT148600.08.5084694
TCGTGTA130900.08.36630694
AGAGCGT256200.08.29233594
AGCGTCG186600.08.1863394
ATCGGAA421950.08.16508194
AGATCGG509750.08.13258994
GCGTCGT165450.07.755563394
TCGGAAG441100.07.682732694
CGTCGTG161250.07.578637694
AAGAGCG311100.07.50885894
TAGATCG163800.07.4319694
CGTGTAG138400.06.928058694
CGGAAGA423350.06.83909594
GAGCGTC253850.06.55456194
AAGATCG217450.05.96577994
CAGATCG166550.05.30555494
CATACGA33150.05.24567314-15
Produced by FastQC (version 0.11.5)