FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39553632
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG925900.012.64951694
ATCGGAA978800.011.0871594
CGGAAGA828050.010.63092894
GATCGGA1061400.010.60958192-93
ACACGTC336500.09.34396194
AGATCGG1169600.08.70385590-91
TAGATCG272850.08.31120290-91
CACACGT410650.08.20610994
CAATTCT334450.07.8690191
CGATTCT191900.07.85081961
CAGATCG336900.07.49141390-91
CTACTCG193300.07.3068371
GCACACG480600.06.913948592-93
CACGTCT351500.06.7924894
CTAATTT860650.06.59721661
GAGATCG805600.06.484559590-91
TACTCGG208400.06.48371932
ACGTCTG307900.06.380526594
CGTCTGA253550.06.339462894
CTAGTTT145700.05.9779491
Produced by FastQC (version 0.11.5)