FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35015189
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA501250.08.48569794
ATCGGAA467600.07.9002694
TAGATCG189750.07.802290494
AGATCGG562150.07.66673594
TCGGAAG480000.07.255549494
ACACGTC198150.07.18690594
CGGAAGA464700.06.938167694
GCACACG287400.06.492277694
CGTCTGA153150.06.413906694
CACACGT244050.06.1433594
ACGTCTG196550.05.93023694
CAGATCG169700.05.7883994
CACGTCT223200.05.72753994
AGAGCAC462750.05.4439294
AAGATCG214800.05.36074594
ACGACTA44400.05.081028594
Produced by FastQC (version 0.11.5)