FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20998104
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC201750.09.66779894
AGATCGG669450.08.58620794
GATCGGA601250.08.457952594
TCGGAAG540800.08.41262894
ATCGGAA560950.08.194221594
CACACGT236950.08.17209794
CGTCTGA140800.08.11141694
CGGAAGA512200.08.05652994
TAGATCG204450.07.99991194
GCACACG277500.07.85865294
CACGTCT202400.07.755842794
AAGATCG236350.07.73547694
AGAGCAC433150.07.150561394
ACGTCTG178100.07.12513294
AGCACAC369400.06.908694394
CAGATCG185050.06.55275194
GAAGAGC559800.06.44794594
TCGCGTA5507.057647E-56.410279342-43
CGCGTAT6053.9077593E-56.216250444-45
AAGAGCA539150.06.189303494
Produced by FastQC (version 0.11.5)