FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33872173
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA10300.015.28900842-43
CGCGTAT11250.014.62515444-45
TATGCCG35750.011.56943248-49
GCGTTAA37750.011.01829636-37
CGTATGC38950.010.74068946-47
AATCGCG15000.010.49839140-41
ATCGCGT15200.010.20568542-43
CGTTAAT43600.09.32433236-37
ACACGTC354950.08.805325594
ATCGGAA1007900.08.67334294
TAGATCG376300.08.58051894
TCGGAAG962900.08.566174594
ACGTCTG317150.08.50624994
GATCGGA1088200.08.49113594
CGTCTGA254850.08.48328794
AGCGTTA48500.08.28537534-35
CACACGT422350.08.20136294
AGATCGG1198300.08.20123994
CGGAAGA903500.07.99533594
TCGTATG33200.07.858502444-45
Produced by FastQC (version 0.11.5)