FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34999327
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT383500.010.15882594
CGTCGTG354850.09.87980494
AGCGTCG436950.09.56146394
TCGTGTA275250.09.39052194
GATCGGA1113950.09.15900894
GTCGTGT313150.09.01937494
CGTGTAG267150.09.0067794
AGAGCGT572000.08.94717994
GAGCGTC528100.08.93453294
AGATCGG1245000.08.66298694
AAGAGCG645350.08.60748994
TCGGAAG979950.08.56029394
ATCGGAA1022400.08.54501594
TAGATCG408700.08.50905694
CGGAAGA906150.08.25652594
GAGATCG550300.07.156469394
AAGATCG413600.07.03338694
GTGTAGG323550.06.87027494
GAAGAGC944550.06.7615994
CAGATCG311450.06.729779294
Produced by FastQC (version 0.11.5)