FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35139176
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT222300.09.38653594
ATCGGAA610150.09.11191994
GATCGGA662700.09.02761894
AGCGTCG259750.09.01022394
CGTCGTG212500.09.00113594
AGATCGG749800.08.88149794
AAGAGCG395900.08.606256594
TCGGAAG601100.08.57672994
GTCGTGT197150.08.55774894
GAGCGTC329100.08.4395994
TAGATCG246700.08.43910494
TCGTGTA169700.08.391170594
AGAGCGT347450.08.10207694
CGGAAGA570100.07.93847494
CGTGTAG172650.07.54006394
AAGATCG278050.07.30167794
CAGATCG221850.06.18564894
GAGATCG386350.06.17937594
GAAGAGC670300.05.90343394
GTGTAGG241600.05.64108894
Produced by FastQC (version 0.11.5)