FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35139176
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT10750.014.64940644-45
TCGCGTA11450.013.34275442-43
AATCGCG14300.011.34091940-41
CGTATGC37100.010.7061346-47
TATGCCG38400.010.46499948-49
ATCGCGT16650.010.02263242-43
TAGATCG228400.09.11602494
ATCGGAA623850.08.95776294
GATCGGA671950.08.92506794
AGATCGG747600.08.87065294
TCGGAAG617900.08.43550694
CGGAAGA587200.07.691926594
ACACGTC240850.07.64957394
CGTCTGA179200.07.4486694
TCGTATG33150.07.090390744-45
CTCGTAT38100.07.032885644-45
CACACGT294100.06.96769794
AAGATCG293300.06.81043294
CAGATCG214450.06.728374594
GCACACG340100.06.578064494
Produced by FastQC (version 0.11.5)