FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36467174
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT245100.010.0471194
AGCGTCG282650.09.54368694
GTCGTGT212800.09.20910994
AGAGCGT383250.09.08634694
GATCGGA701800.09.02001994
CGTCGTG235150.08.67357294
AGATCGG788300.08.65025994
TAGATCG257600.08.64740394
ATCGGAA643000.08.51467794
GAGCGTC354550.08.36382894
TCGTGTA182500.08.34325294
AAGAGCG433800.08.15752994
TCGGAAG644450.07.99964394
CGTGTAG185100.07.769055494
CGGAAGA609950.07.41968294
AAGATCG289600.06.84805394
CAGATCG231100.06.771696694
GAGATCG408650.06.267555794
GAAGAGC722700.06.073541694
GTGTAGG254200.05.601705694
Produced by FastQC (version 0.11.5)