FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32150078
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT113700.010.16806294
GATCGGA357500.09.47813394
TCGTGTA92250.09.32284194
ATCGGAA333750.09.29364994
AGATCGG411350.09.21988794
AGCGTCG131800.09.19957794
TCGGAAG344750.08.58815494
AGAGCGT195750.08.2828794
GAGCGTC188000.08.02436694
AAGAGCG225850.07.99051494
TAGATCG134350.07.975551694
CGGAAGA336100.07.858350394
CGTCGTG116150.07.586572692-93
GTCGTGT108950.07.37619694
AAGATCG169700.06.701886794
CAGATCG148350.06.49426394
CGTGTAG102000.05.34467692-93
GAAGAGC457550.05.29998694
GAGATCG278300.05.28560594
Produced by FastQC (version 0.11.5)