FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33377920
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG141050.010.79513494
GATCGGA375150.010.22212794
GCGTCGT116450.010.0488494
AGATCGG430600.09.23320394
TAGATCG140900.09.1722994
ATCGGAA348800.09.09459594
AAGAGCG233000.08.55196794
AGAGCGT203900.08.50482294
CGTCGTG116250.08.48950694
TCGGAAG363450.08.4176894
CGGAAGA351200.08.21617994
AAGATCG180400.07.65890894
GAGCGTC199350.07.520219394
TCGTGTA96500.07.061496794
GTCGTGT112450.06.895734394
CGTGTAG108450.06.67340194
GAGATCG297150.05.978223394
CAGATCG159350.05.632969494
Produced by FastQC (version 0.11.5)