FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21620116
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG141350.09.50894494
TCGTGTA95450.09.35491394
GATCGGA364300.09.12057794
GCGTCGT123700.09.08009294
TAGATCG138300.08.83513594
CGTCGTG121100.08.73173294
ATCGGAA338450.08.70632894
AGATCGG410350.08.623878594
AGAGCGT195500.08.6059494
AAGAGCG223100.08.57347594
GAGCGTC184400.08.41036994
GTCGTGT110000.08.37385594
TCGGAAG334250.08.25332294
CGTGTAG96800.08.20490294
CGGAAGA321300.08.04477794
AAGATCG153150.07.088544494
GAGATCG227500.06.486498494
CAGATCG120900.06.37499294
GAAGAGC384150.05.66424794
GTGTAGG141650.05.474310494
Produced by FastQC (version 0.11.5)