FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22417918
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT133400.010.18115194
AGCGTCG153550.09.27358594
GATCGGA385500.09.02115694
ATCGGAA357250.08.971539594
AGATCGG436000.08.84935394
TAGATCG146300.08.83371394
GAGCGTC197100.08.72668694
CGGAAGA339600.08.41377394
TCGTGTA102750.08.36997494
AAGAGCG231600.08.33984894
CGTCGTG126850.08.33579394
GTCGTGT115500.07.934279494
TCGGAAG353150.07.87802594
AGAGCGT207550.07.54008794
CGTGTAG106350.07.2028694
AAGATCG164450.07.172899294
CAGATCG129450.06.13535294
GAGATCG239950.05.993155594
GAAGAGC405150.05.567756794
GTGTAGG151600.05.36292594
Produced by FastQC (version 0.11.5)