FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31408683
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT254650.09.11672994
AGAGCGT386250.09.06449394
AGCGTCG292700.08.95915994
ATCGGAA671150.08.88582594
CGTCGTG241850.08.82196994
TCGTGTA190800.08.57149794
AGATCGG815950.08.558763594
GAGCGTC357700.08.42160494
GATCGGA726850.08.41178794
CGGAAGA619000.08.36655594
CGTGTAG194000.08.16364294
GTCGTGT219550.08.13403394
AAGAGCG437950.08.037353594
TCGGAAG654800.07.99525494
TAGATCG253400.07.956213594
AAGATCG281750.07.138970494
GAAGAGC687650.06.027773494
GAGATCG412400.05.93710694
CAGATCG226500.05.851088594
GTGTAGG252700.05.70937994
Produced by FastQC (version 0.11.5)