FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30913750
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA8950.08.92888842-43
AATCGCG13700.08.92116340-41
ATCGGAA862200.08.55284494
CGCGTAT10050.08.41965144-45
TAGATCG300600.08.31798794
GATCGGA926700.08.17056394
AGATCGG1019250.08.13417494
TCGGAAG827750.07.960575694
ACACGTC330550.07.80604194
ACGTCTG294050.07.784010494
CACACGT388200.07.712221694
GCACACG446300.07.63495994
CGGAAGA781250.07.501913594
TATGCCG32800.07.4513548-49
CACGTCT333050.07.43698394
CGTATGC33650.07.403618346-47
CGTCTGA239200.07.368273394
AAGATCG349600.06.816040594
CAGATCG268350.06.69048194
AGCACAC579750.06.663872794
Produced by FastQC (version 0.11.5)