FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34536299
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT12450.013.02684444-45
TCGCGTA12300.012.61196842-43
AATCGCG15050.011.24446440-41
TATGCCG37700.011.1579848-49
CGTATGC39050.010.71323646-47
CGTCTAA50100.09.709434536-37
ATCGCGT16750.09.401648542-43
AGATCGG1049350.08.46076494
GATCGGA954400.08.42592394
TAGATCG318000.08.276731594
ACACGTC334900.08.23798494
TCGGAAG858900.08.12062194
ATCGGAA883950.08.01810294
TCGTATG34650.07.868902744-45
CCGTCTA62450.07.676409234-35
CGTCTGA246500.07.62677694
GCACACG456750.07.408868394
CACACGT392950.07.403742394
CGGAAGA812200.07.360749794
CACGTCT341950.07.29843794
Produced by FastQC (version 0.11.5)