FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35797991
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT360500.09.25556694
TCGTGTA265800.09.15850694
AGCGTCG409050.09.11059394
CGTCGTG339850.08.93295494
AAGAGCG602450.08.76789194
GATCGGA989800.08.5604894
GAGCGTC499850.08.55562194
GTCGTGT300450.08.415112594
TCGGAAG882700.08.34800694
CGTGTAG261450.08.17848194
ATCGGAA912200.08.1450194
AGAGCGT528450.08.083693594
AGATCGG1097950.07.995473494
CGGAAGA826300.07.8087794
TAGATCG349700.07.47186594
AAGATCG384350.06.70044394
CAGATCG295950.06.685187394
GTGTAGG317400.06.64797394
GAAGAGC896900.06.29811194
GAGATCG496050.05.835863694
Produced by FastQC (version 0.11.5)