FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28536294
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG15500.017.43873640-41
CGCTCGA49950.014.72516834-35
CGAATCG19900.013.933681538-39
TCGAATC54500.013.62471938-39
ACGCTCG61000.012.17330934-35
CTCGAAT70200.010.845695536-37
GCTCGAA72350.010.29604136-37
CGAATCT50600.09.6593938-39
ATCGCGG29000.09.2397142-43
ATCGGAA679000.08.72860994
GATCGGA735100.08.72742194
AGATCGG806650.08.60587594
TAGATCG250750.08.41598794
ACACGTC257300.08.25654494
TCGGAAG657400.08.15747394
CGGAAGA624600.07.931188694
CACACGT306850.07.857617494
ACACGCT106600.07.517069332-33
TCACACG106250.07.29838130-31
CGTCTGA186750.07.24822394
Produced by FastQC (version 0.11.5)