FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29628309
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT225250.09.72240194
AAGAGCG459200.09.34375194
AGAGCGT398250.09.22790994
GAGCGTC370150.09.20476394
GCGTCGT264100.09.18192494
AGCGTCG299700.09.07912994
TCGTGTA190500.09.07831594
GATCGGA764100.08.78274794
CGTCGTG250000.08.75988394
ATCGGAA703950.08.43167794
CGTGTAG190550.08.28672194
CGGAAGA642900.08.2382194
AGATCGG850550.08.1331894
TCGGAAG681300.07.93253294
TAGATCG276850.07.68964194
AAGATCG277250.06.475065794
CAGATCG235800.06.437409494
GAAGAGC704300.06.098750694
GGAAGAG941450.05.92024794
GTGTAGG241950.05.90473494
Produced by FastQC (version 0.11.5)