FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33150632
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA11400.016.28781542-43
CGCGTAT11950.015.93217544-45
TATGCCG36900.013.69286648-49
CGTATGC40000.012.28043646-47
ATCGCGT18250.010.8182542-43
CTCGTAT35550.09.65319744-45
TCGTATG32700.09.416326544-45
TAGATCG244650.08.66417594
AGATCGG768900.08.57597894
ATCGGAA656650.08.47449294
GATCGGA697750.08.37273294
ACACGTC256550.07.950850594
TCGGAAG645400.07.89398394
CGGAAGA617000.07.31277194
CACACGT308450.07.28348194
CGTCTGA193650.07.256873694
GCACACG358500.07.18434794
ATGCCGT75250.07.12050248-49
ACGTCTG242150.06.851507794
AAGATCG296750.06.778739594
Produced by FastQC (version 0.11.5)