FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36932406
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT15450.020.23450344-45
TCGCGTA15100.020.08011642-43
CGTATGC47550.017.84348146-47
TATGCCG52950.016.2437748-49
ATCGCGT21800.014.7712642-43
TCGTATG39250.014.01346644-45
CTCGTAT44850.012.94505444-45
ATGCCGT95300.09.34583548-49
GCGTATG34000.09.33306444-45
TCTCGTA67000.09.26152742-43
TAGATCG235000.08.7399994
AGATCGG796300.08.711781594
GCCGTCT100950.08.68314250-51
ATCGGAA675250.08.57518494
GATCGGA723800.08.55193894
GTATGCC119000.07.82115946-47
CCGTCTT112800.07.812390352-53
TCGGAAG670300.07.677896594
ATCTCGT83050.07.641482442-43
TGCCGTC116100.07.570311550-51
Produced by FastQC (version 0.11.5)