FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38211580
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG247350.09.04371194
GCGTCGT257300.09.02274694
GATCGGA749550.08.94694994
TCGTGTA194200.08.88113494
ATCGGAA691450.08.65885194
AGAGCGT395100.08.63537594
AAGAGCG453950.08.62359994
AGCGTCG293950.08.61721494
GTCGTGT221900.08.53491894
TAGATCG262150.08.51520994
AGATCGG838550.08.51295794
GAGCGTC365900.08.39976194
TCGGAAG676900.08.29650194
CGGAAGA646650.08.17588694
CGTGTAG202100.06.88298894
AAGATCG306450.06.80886894
CAGATCG256500.06.48585994
GAAGAGC748500.05.86417294
GAGATCG446300.05.528203594
GTAGGGA244900.05.334670594
Produced by FastQC (version 0.11.5)