FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28002321
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG285850.010.489294
GCGTCGT310500.010.42839594
AGCGTCG357700.010.33988394
AGAGCGT472900.09.6496194
GTCGTGT249300.09.53867694
AAGAGCG537700.09.38694194
TCGTGTA218900.09.27465694
GAGCGTC435150.09.0071494
CGTGTAG210600.08.97072494
ATCGGAA849000.08.88991794
GATCGGA925950.08.73480494
CGGAAGA759750.08.46822894
TCGGAAG808550.08.17799994
AGATCGG1025900.08.14491694
TAGATCG328600.07.66579394
GTGTAGG258350.07.221740794
AAGATCG340750.07.04765994
GAAGAGC779900.07.00810694
CAGATCG258750.06.84732394
GAGATCG454450.06.38056294
Produced by FastQC (version 0.11.5)