FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29051394
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA228350.010.8252594
GCGTCGT327650.010.54217994
CGTCGTG303850.010.03780394
GTCGTGT263350.09.88620794
AGAGCGT498800.09.79851194
AGCGTCG377050.09.68445794
GAGCGTC457850.09.22761994
AAGAGCG561350.09.06667394
CGTGTAG223250.09.05170194
GATCGGA975650.08.84365794
CGGAAGA799250.08.74930494
ATCGGAA894500.08.59520394
AGATCGG1083950.08.35026594
TAGATCG352350.08.22932294
TCGGAAG858700.08.09431294
AAGATCG356400.07.832527694
GTGTAGG268500.07.088656494
CAGATCG274200.06.99271694
GAAGAGC810900.06.902360494
GTAGGGA225250.06.82238494
Produced by FastQC (version 0.11.5)