FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35771618
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT11400.011.75211544-45
TCGCGTA12400.010.61449542-43
TATGCCG39550.09.09118748-49
CGTATGC38900.08.88145346-47
GATCGGA784000.08.53652994
AGATCGG871600.08.42269794
TAGATCG269050.08.38485694
ATCGGAA735000.08.235991594
TCGGAAG716000.08.04757194
ATCGCGT17600.07.87902242-43
CGTCTGA210600.07.766195394
CGGAAGA689100.07.68650894
ACACGTC286200.07.652506494
CACACGT341700.07.303694
ACGTCTG267400.07.048072394
AAGATCG328950.06.758007594
CACGTCT306100.06.64832194
GCACACG397050.06.616891494
TCGTATG33150.06.523044644-45
CAGATCG238700.06.32033494
Produced by FastQC (version 0.11.5)