FastQCFastQC Report
Fri 27 May 2022
EGAF00005407121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100553285
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC18382261.8281113342045463No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA4004680.39826446246882935No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC3575960.355628361619414No Hit
GCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT3395350.33766674057441287No Hit
GTGGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGT3341020.3322636351462809No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG3101300.30842353882322193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2451370.2437881566972178No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2425630.241228319890295No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG2319700.23069360687718957No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG2239260.22269386823115725No Hit
CCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCC1827990.18179316568324944No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC1785010.17751881502429284No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1752390.17427476387270688No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1697570.16882292806246957No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1672430.16632276111118596No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC1671940.16627403072908062No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1623860.1614924862971906No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1596460.15876756288966593No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1573660.15650010837537529No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1569430.15607943589311876No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1561190.1552599698756734No Hit
ACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGC1542780.15342909980514313No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG1530220.15218001082709529No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1484460.1476291898370103No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT1383140.1375529402147329No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC1372100.13645501487097114No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC1359940.13524570579668282No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA1342020.13346356610825794No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT1281640.12745878963576376No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC1276480.1269456288772664No Hit
CTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACG1256550.1249635951724501No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG1245950.12390942772282378No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1239500.12326797677470208No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA1224590.12178518086206731No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1203280.11966590648928078No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1184110.1177594546016075No Hit
CCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCC1162270.11558747185633966No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG1149870.11435429483979563No Hit
CTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC1125850.11196551161903862No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1040620.10348940862548647No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA1039980.1034257607794713No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT1030090.10244220265901804No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)