FastQCFastQC Report
Fri 27 May 2022
EGAF00005407189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86356904
Sequences flagged as poor quality0
Sequence length25-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC2244200.25987499505540407No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1313440.15209438263326347No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1153990.13363031171196224No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC914700.105920888502441No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC913100.10573561090147467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)