FastQCFastQC Report
Fri 27 May 2022
EGAF00005407237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106834782
Sequences flagged as poor quality0
Sequence length25-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA10689931.0006039044475235No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG9889470.9256788673935799No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG8712960.8155546196556099No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG7848070.7345987751442222No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG7295440.682871239443349No Hit
CCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCC5767890.5398887789184612No Hit
ACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGC5540440.5185988960037379No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC5155390.4825572630456624No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC4553390.4262085731592545No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG4330200.40531743678758103No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT3883800.36353329199473633No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC3717910.34800557743451005No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA3713060.3475516054312724No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC3583160.3353926439424943No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC3455810.32347236876469687No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC3370530.3154899497057054No Hit
CCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGA3130660.293037524052794No Hit
CGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAG2900280.27147338588663006No Hit
ACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGC2883590.26991116058064313No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC2881130.26968089849240295No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG2670800.24999348994787107No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG2664990.24944965956873486No Hit
GACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGG2505040.23447794370938108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2451670.22948237962426882No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC2429190.22737819598864345No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG2413250.22588617253882728No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG2277770.21320490924013866No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA2202780.20618565964781022No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC2192350.20520938583466194No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG2114080.19788312012467998No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTGC1958910.18335882409532134No Hit
GCCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGC1940020.18159067334456677No Hit
GGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCG1806030.16904887773347074No Hit
CGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGCG1775430.16618464200170316No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC1747710.16358998139763134No Hit
CGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGC1675680.1568477951309902No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1674030.1566933510474145No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1661300.15550179154200922No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1609840.15068500818394517No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1609330.15063727092174906No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1583230.14819424632700612No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1567360.14670877505043256No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1503050.1406891998899759No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1492370.1396895254581041No Hit
CCTCTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAA1412870.13224812870400204No Hit
CCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGG1385500.12968622896614326No Hit
GCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGA1342460.12565757844668976No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC1303170.12197993720809017No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1302980.12196215273786024No Hit
GCACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC1294270.1211468751815303No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG1282500.12004517405202363No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1279200.11973628588487222No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTG1275390.11937966045552469No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1274190.11926733748565145No Hit
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGC1269360.11881523753191166No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC1249890.11699279734571837No Hit
CCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCA1243900.11643211852110111No Hit
GGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGG1239290.11600061111183808No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1217740.11398347777786452No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1204630.11275634933199938No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT1196480.11199348916161031No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG1171280.1096347067942723No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC1169490.10946715836421138No Hit
CCTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTG1165490.10909274846463393No Hit
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCG1151250.10775984922213816No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA1143930.10707467910591141No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA1116640.10452026756604417No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG1115330.10439764832393256No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA1104420.10337644532283502No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC1094960.10249096591033434No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1094390.10243761249964455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)