FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17201406
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAAGTTTCAGCTTTGCAACCAT358810.2085934138174519No Hit
TGCCCTCCAATGGATCCTCGTTAAAG331750.19286214161795845No Hit
CGTTCGTTATCGGAATTAACCAGACA309730.18006086246670766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG64300.018.0075021
GAATGAA57250.017.8093031
ATGCGAT43100.017.6133331
CCTTACG66500.017.4724861
CGGAGCT40000.017.3631881
GGAACTT60800.017.2177411
GATGAGG81400.017.1761721
GAACCTA50700.017.0637261
GATCGCG43650.016.9751191
GGGATGA57200.016.942451
GCGCGAT31350.016.9052961
CCTCTGA61700.016.9011481
GCGGGTT90450.016.8526951
GGACATT118400.016.7878481
CACCGAA43700.016.787788
CTGTGCT83850.016.746541
TAAGTGC94700.016.7359641
CCGAATT54050.016.66125710
CTGCCTA55150.016.6570051
TGATTTC49750.016.6387921
Produced by FastQC (version 0.11.5)