FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34463555
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG122700.017.8771131
CGATTGA130750.017.8722521
GAGCAGA100850.017.648391
TGAGCCG155850.017.5964491
CAATGAA125500.017.5457428
CCGGGAT88700.017.392671
CCTACCA160800.017.3810541
TGGGCGT113500.017.2993341
GCTCCTA175400.017.2400911
ACGAGCC94650.017.226741
GACCTGG117900.017.1843191
GGAACTT124950.017.1433681
GCGCGAT88700.017.1196651
GACTAAC89450.017.0776421
ATGCGAT87100.017.0750391
ATGAATG102350.017.02199610
CGACCTT106000.017.0193941
GGGCATC114850.016.9905661
ACTGAAC89400.016.985621
CGGAGCT101000.016.9728361
Produced by FastQC (version 0.11.5)