FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40965471
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTGG189200.017.9682371
GGGACCT147650.017.8913351
ACTGATG155450.017.8712371
ATAGACC128600.017.6340661
ATGCGAT129550.017.6061631
CGGAGCT127650.017.5673831
CCGGGAT106350.017.3608781
CTGTGCT171300.017.1969641
GAACCTA128700.017.1806431
GCGCGAT91150.017.1626721
CAGCCTT170200.017.139948
TGAGCCG115800.017.1135311
AAACCTG142650.017.0874041
CCTCTGA136550.017.0144481
TACCTAT211300.016.9976371
TGGGCGT131300.016.9558811
TCCGCGA91950.016.90110210
CACACAA145600.016.8800141
GGGAATG111000.016.8794141
CGTTGGG168450.016.8399961
Produced by FastQC (version 0.11.5)