FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26588133
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT100450.018.0209961
CCACCTA162800.017.8317741
ACTGATG83150.017.7546221
GAAACTC124750.017.6172311
CCGGTAG105400.017.4338631
TACTTGT112300.017.4258961
CTAAGAC86600.017.386151
GTAACTG113300.017.3792611
TACTTAC135450.017.2863271
TGGACGC81900.017.2843151
TGCGCAG125700.017.282841
CCACTAC85600.017.2346361
CGGACTG81800.017.2312281
ATGCGAT69500.017.194261
GGGCATC101750.017.1841351
GATGCTA96800.017.174361
TCTGGAA94750.017.0760571
CGGAGTC117850.017.0688861
TATGCCC78850.017.0546041
TGAGCAT109300.017.024721
Produced by FastQC (version 0.11.5)