FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53714610
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGT250000.018.0600031
GGAACTT262950.017.9645391
ATGCGAT162900.017.7248061
ATAGACC162350.017.6787321
GACCAAT219150.017.5501351
CCCTCCT277100.017.2556571
TGACTAG169700.017.2235851
GCGCGAT122100.017.1899471
TGCCAAA196650.017.1668571
CTCGAAA164950.017.0928691
GAATGAA176650.017.0450841
CGATGGC232150.017.0344731
ACGAGGA165050.016.9597031
GGGTTGC228600.016.9576381
CGCTGGA175850.016.9266761
TGCACCT229350.016.8712521
CAACTAG229750.016.85071
CGACCTT158800.016.7783871
TGACGGC145400.016.714561
GGGAGAT162700.016.712571
Produced by FastQC (version 0.11.5)