FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30461503
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC142050.018.066111
CGGACTG117300.017.8869861
GGCTCGA104650.017.4723591
GGATTAC113100.017.363521
CCGTACT113950.017.3043771
CGACCTT130450.017.1981581
GGTATTG146750.017.0981331
TTAGGCA125900.017.07921
TTTATGC112700.017.0693511
CGGACAC103650.017.0316221
GGGACCT86500.017.0128021
GGGAATG92600.016.9963071
AGCTTGA122500.016.9313011
TATTGAG75550.016.9267963
ATGCGAT83250.016.918351
CGTGAGC98300.016.8775881
ACTGATG77650.016.87331
TGGCCAG109700.016.8507881
AAACCTG90700.016.7997261
CACCCAT93100.016.7834938
Produced by FastQC (version 0.11.5)