FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50157587
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT166650.017.5151481
ACTGATG166150.017.4889141
GACTAAC131600.017.2043271
TGACAAC177750.017.0684661
GATGCTA155200.016.9025541
CAATGGA183950.016.8718368
AGTGAGG202600.016.8424341
CCGGGAT112350.016.748521
GCGCGAT120850.016.6725541
CTGATCC186850.016.6475811
ATAACGC139350.016.558841
CTGATAG162450.016.4401191
TGAGCCG146500.016.4324971
GAACCTA151900.016.3464891
CGACCTT147000.016.3422851
TGGTTAG183550.016.3422261
ACTGAAC126750.016.3342841
CGCTATC154850.016.3282851
TCTAACT161500.016.2711648
TCCCGAT135000.016.2478711
Produced by FastQC (version 0.11.5)