FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12628269
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG56550.018.4117951
GAACCTA84150.018.3245531
TCTATTG89700.018.0158231
CTGAAGT53100.018.0042671
GGGACCT34250.017.6418421
CAAGGAC64800.017.609978
TCCCGAT53100.017.6033231
GCTGCGA52800.017.5881371
ATGCGAT44750.017.5803281
TGGACGC39800.017.5761721
TGATTTC61350.017.5661661
CGGACGT31950.017.4839271
CCGTACT47000.017.4721151
TGCGTGG44650.017.460761
TGCGGGT54050.017.4439771
CTAATGG43550.017.3896451
TATATGA38950.017.3679589
ACACCCT53000.017.349611
CCTATTA55050.017.3296811
CGCTGGA45350.017.3253771
Produced by FastQC (version 0.11.5)