FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12694792
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG55850.018.1334671
GAACCTA80450.018.1150171
TCTATTG88050.018.0351891
GCTGCGA55750.017.8753361
CTGAAGT54000.017.8357241
TGAGCAT56900.017.7988411
CCGTACT45600.017.7898251
TGATTTC62400.017.6745051
CGCTATC58350.017.6689571
AACCTAA42050.017.6606862
GCGATCA53800.017.55314
TAGCTAA48800.017.528849
GAAGCAG56200.017.515961
CGCTGGA52500.017.4965321
CAAGGAC63250.017.4740588
GCACATA60750.017.471051
CTGCGAT40250.017.4571822
CGAACAT55650.017.452491
CGGACGT33150.017.4138721
AAGTCGA27950.017.3761546
Produced by FastQC (version 0.11.5)