FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005407491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12694792
Sequences flagged as poor quality0
Sequence length98
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT498510.392688592298322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110600.049.806289
TGGTATC113350.047.6996467
GGTATCA118000.046.601968
AAGCAGT144050.040.551561
GTGGTAT145950.037.4232676
AGCAGTG154150.037.402192
CAGTGGT154500.035.621634
AGTGGTA154700.035.3080945
ATCAACG106750.030.75539210-11
CAACGCA124250.027.55314812-13
AGAGTAC112650.026.8195618-19
ACGCAGA127250.026.8116414-15
TCAACGC116150.026.01052312-13
GCAGTGG244800.023.9467683
AACGCAG131350.023.78683314-15
GCAGAGT141100.021.97754516-17
CGCAGAG140650.021.93345316-17
TATCAAC130250.021.81733310-11
GTACTTT120600.021.58595722-23
GAGTACT142150.019.0250520-21
Produced by FastQC (version 0.11.5)